Quick Overview for SlyD (AA 1-196) (Active) 蛋白 (ABIN667072)
抗原
SlyD
蛋白类型
Recombinant
生物活性
Active
宿主
E. coli
资源
大肠杆菌（E. Coli）
应用范围
SDS-PAGE (SDS), Enzyme Activity Assay (EAA)
纯度
> 95 %
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产品特性
- AA 1-196
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序列
- MKVAKDLVVS LAYQVRTEDG VLVDESPVSA PLDYLHGHGS LISGLETALE GHEVGDKFDV AVGANDAYGQ YDENLVQRVP KDVFMGVDEL QVGMRFLAET DQGPVPVEIT AVEDDHVVVD GNHMLAGQNL KFNVEVVAIR EATEEELAHG HVHGAHDHHH DHDHDGCCGG HGHDHGHEHG GEGCCGGKGN GGCGCH
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产品特性
- SlyD,1-196aa, E.coli, Recombinant, E.coli
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纯化方法
- > 95% by SDS-PAGE
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Biological Activity Comment
- Specific activity is > 700nmol/min/mg, and is defined as the amount of enzyme cleaves 1nmol of suc-AAPF-pNA per minute at 37C in Tris-HCl pH 8.0 using chymotrypsin.
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SlyD (AA 1-196) (Active) protein
宿主: E. coli 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % pure SDS Active
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- 20 mM Tris-HCl buffer ( pH 7.5)
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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- SlyD
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背景
- SlyD is a putative folding helper protein from the Escherichia coli cytosol, which consists of an N-terminal prolyl isomerase domain of the FKBP type and a presumably unstructured C-terminal tail. It is involved in the biosynthesis of the metal cluster in the [NiFe]-hydrogenase enzymes, and exhibits several activities including that of a peptidyl-prolyl isomerase. Recombinant E. Coli slyD was expressed in E. coli and purified by conventional chromatography.
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分子量
- 21 kDa (196aa) confirmed by MALDI-TOF
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NCBI登录号
- NP_755987
抗原
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